In vitro Pharmacology

  • Radioligand binding assay on GPCR, nuclear receptor and non receptor targets (eg. ion channels)
    • Equilibrium binding parameters (IC50, Ki, Kd, Bmax)
    • Radioligand kinetic parameters (κonκoff), radioligand binding reversibility)
    • Un-labeled ligand dissociation kinetics
    • Allosteric interactions
    • Mathematical models for complex radioligand/receptor interactions
  • Enzyme activity assays using homogeneous and heterogeneous tecniques: Km, Vmax, IC50
  • Functional assays (eg. gene reporters, second messengers): EC50, pKb, pA2, positive/negative modulation
  • Microbiology: MIC and MCF

 

 

NiKem Research Services: Focused on Drug Discovery

Our Services: in vitro Screening

Biochemical Pharmacology

NiKem Research offers a wide range of methods for target profiling of our clients compounds. Our team has many years of experience of supporting a wide range of drug discovery programmes.
Our in vitro pharmacology service is supported by a cell culture facility capable of performing large cells scale-up. We can assay using tubes, 96 or 384 well plate formats and use human recombinant cell lines or naïve/treated tissues.
These services are available standalone or as part of a fully integrated drug discovery service.

For more information and prices please contact: info@nikemresearch.com